應用血液生化多態型估計不同毛色豬種之遺傳距離

林德育(1) 趙俊炫(2) 張明雄(2) 李啟忠(1) 吳松鎮(1)
曾晉郎(1) 李 力(2) 張秀鑾(1) 吳明哲(1)

(1)台灣省畜產試驗所 (2)國家實驗動物繁殖及研究中心

本試驗採集台灣省畜產試驗所台東種畜繁殖場蘭嶼豬保種族群及選育族群各45及29頭黑色蘭嶼豬、30頭棕白條紋相間迷彩豬(杜洛克種與蘭嶼種雜交選育成)與28頭白色藍瑞斯豬的頸靜脈血樣,以醋酸纖維電泳法分析血漿中澱粉炊@型(AMY1)、澱粉炊G型(AMY2) 與解脂物(ES),及紅血球膜酵素型之葡萄糖磷酸基異構物活]GPI)及6-磷酸鹽葡萄糖去氫物活]PGD),藉以瞭解同一飼養場所的豬種血型之遺傳變異及其類緣關係。結果顯示蘭嶼豬選育族群中GPI與PGD皆為單一型,蘭嶼豬保種族群之GPI亦為單一型。異質結合率在藍瑞斯、迷彩豬、蘭嶼豬選育族群與蘭嶼豬保種族群分別為0.42b0.05(標準機差)、0.46b0.07、0.31b0.13和0.31b0.12。四個族群之類緣關係以蘭嶼豬選育族群與蘭嶼豬保種族群之遺傳距離最近(0.004),藍瑞斯與蘭嶼豬保種族群之遺傳距離為0.018,藍瑞斯與蘭嶼豬選育族群之遺傳距離為0.013,藍瑞斯與迷彩豬之遺傳距離為0.253,迷彩豬與蘭嶼豬保種族群之遺傳距離為0.438,迷彩豬與蘭嶼豬選育族群之遺傳距離為0.465。黑色豬種與白色豬種之遺傳距離較與紅棕色豬種還近。

關鍵語:豬、多態性、遺傳距離。

 

ESTIMATION OF GENETIC DISTANCE AMONG BREEDS WITH DIFFERENT
COAT COLOR BY BLOOD BIOCHEMICAL POLYMORPHISM IN PIGS

D. Y. Lin(1), J. S. Chao(2), M. H. Chang(2), C. J. Lee(1), S. C. Wu(1), J. L. Tzeng(1),
L. Lee(2), H. L. Chang(1) and M. C. Wu(1)

(1)Taiwan Livestock Research Institute
(2)National Laboratory Animal Breeding and Research Center

Genetic variation and distance on blood biochemical polymorphism among breeds of pigs: black Lanyu in conservation, black Lanyu in selection, brown-white stripping Mitsae (derived from hybrid of Duroc and Lanyu), and white Landrace raised at Taitung Breeding Animal Propagation Station of Taiwan Livestock Research Institute, were estimated. Blood samples of pigs from above four breeds were collected from jugular vein and there 45, 29, 30 and 28 head, respectively. Three plasma proteins consisting of amylase-1 (AMY1), amylase-2 (AMY2) and esterase (ES) and two erythrocyte enzymes: glucose phosphate isomerase (GPI) and 6-phosphogluconate dehydrogenase (PGD) were typed in cellulose acetate electrophoresis. Lanyu in selection had monophasic GPI and PGD. Lanyu in conservation had monophasic GPI. Heterozygosities of Landrace, Mitsae, Lanyu in selection, and Lanyu in conservation were 0.42b0.05 (Standard error), 0.46b0.07, 0.31b0.13 and 0.31b0.12. Genetic distances among the above four breeds were estimated and there was a close distance (0.004) between Lanyu in selection and Lanyu in conservation as compared to others. Estimation was 0.018 for Landrace and Lanyu in conservation; 0.013 for Landrace and Lanyu in selection; 0.253 for Landrace and Mitsae; 0.465 for Mitsae and Lanyu in selection; and 0.438 for Mitsae and Lanyu in conservation. Genetic distance between black and white breeds was closer than that of between black and reddish brown breeds.

Key Words: Pig, Polymorphism, Genetic distance.