梅山豬粒腺體DNA D-loop區域的單股構形多態性
與繁殖性狀之相關性

顏念慈(1,3) 林志生(2) 黃木秋(3) 戴謙(4)

(1) 行政院農業委員會畜產試驗所 (2)臺灣養豬科學研究所
(3)中興大學 (4) 成功大學

本試驗的目的是探討依據母系血緣推定之梅山母豬粒腺體DNA D-loop區域SSCP態樣與其繁殖性狀之相關性。於1994年7月兩公三母梅山豬由日本引進台灣，至1999年5月已繁殖141胎。引進之三頭母梅山豬來自兩個母系血緣，Meda和Nehime。利用設計之引子增殖40頭梅山豬之D-loop區域392 bp片段，進行單股構形多態性(Single strand conformation polymorphism, SSCP)分析，分析結果梅山豬粒腺體DNA D-loop區域之SSCP態樣可分出兩種環帶態樣，B和C態樣。Meda和Nehime 的SSCP環帶態樣分別為B和C態樣。所有測試之梅山豬粒腺體DNA D-loop區域單股構形多態性皆符合母系遺傳，故依據系譜推定所有參試梅山母豬之SSCP環帶態樣，將此推定之SSCP環帶態樣與141胎繁殖性狀進行分析，分析結果發現來自SSCP B態樣母豬之仔豬其21日齡體重顯著地比C態樣母豬之仔豬重(p<0.05)，此結果顯示粒腺體DNA D-loop區域之SSCP態樣可成為選拔梅山豬仔豬出生後早期生長速率的有用標誌。

關鍵語: 梅山豬、單股構形多態性、繁殖性狀。

ASSOCIATION OF SSCP POLYMORPHISM OF MITOCHONDRIAL DNA
D-LOOP REGION WITH REPRODUCTIVE TRAITS IN MEISHAN PIGS

N. T. Yen(1,3) C.S. Lin(2) M. C. Huang(3) and C. Tai(4)

(1) Taiwan Livestock Research Institution, COA. (2) Pig Reseach Institute.
(3) National Chung Hsing University. (4) National Cheng Kung University.

The purpose of this study was to investigate the relationship between SSCP band patterns in the D-loop region of mitochondrial DNA (mtDNA) and reproductive traits of Meishan (M) sows. Two male and three female M pigs were imported from Japan to Taiwan on July of 1994, and one hundred and forty one litters were propagated from these foundation female M pigs until May of 1999. The foundation female M pigs originated from two maternal lineages, Meda and Nehime. Using the designed primers, the most polymorphic 392 bp fragment in the D-loop region of pigs mtDNA were amplified from 40 M pigs and applied to single-strand conformation polymorphism analysis. Two types of electrophoresis band patterns, B and C, were identified from SSCP analysis. The SSCP band patterns for Meda and Nehime were B and C types, respectively. Because the SSCP genotypes from 40 sampled M pigs followed maternal inheritance, genotypes of the rested animals in this trail were predicted by pedigree information. From analysis of the reproductive traits of M dams with predicted SSCP band patterns, the average body weight at 21 days of piglets from dams of B type was significant heavier than from C type (p<0.05). This result suggested SSCP polymorphism in mtDNA D-loop region might be a useful maker for selection of growth rate in early postnatal stage of M pigs.

Keywords: Meishan pig, SSCP, Reproductive traits.