白羅曼鵝逢機複製多態性DNA片段指印之分析
林德育(1) 劉瑞珍(1) 陳若菁(1) 葉力子(1) 張秀鑾(1) 戴謙(2)
(1)行政院農業委員會畜產試驗所 (2)國立成功大學生物科技研究所
由種鵝族群中依公鵝性成熟體重及母鵝產蛋數分成高體重 (HBW)、低體重 (LBW)、高產蛋 (HEP)及低產蛋 (LEP) 4 組，共48 隻，利用逢機複製多態性 DNA(RAPD)方法分析白羅曼鵝 DNA 多態性。由蹼靜脈採集 3 ml 血液，以 DNA 萃取套組萃取全血之基因組 DNA，測定並調整適當濃度後。將所得之DNA 作為模板，再以合成之 10 鹽基對 (10bp) 寡核甞酸作為引子，進行聚合𨥨連鎖反應 (polymerase chain reaction, PCR)。PCR 複製後之產物以 1.5﹪瓊脂糖膠體進行電泳分析。利用 328 種不同寡核甞酸序列之逢機引子分析各組鵝隻混合 DNA 及個體 DNA，並以 Gelcompare 影像掃描分析軟體分析其 DNA 片段型，尋求可能與鵝隻性成熟體重及產蛋數相關之遺傳標記。結果顯示檢測所有供試鵝隻DNA所使用之 328 種引子中，有 85% 引子可得到 PCR 產物，41%引子可檢測出具變異性的片段。在引子AC09(890bp)、AE03(690bp) 、AE06(920bp) 、AG10(1350bp,1370bp) 、AL13(1288bp) 可得具性別特異性的片段；而引子AD06(720bp)、AD14(700bp)、AH13(2400bp) 及 AJ15(510bp) 四種引子所產生之特異性DNA片段在高低產蛋數組間之頻率具較大差異。
關鍵語：白羅曼鵝、逢機複製多態性 DNA、多態性。
STUDY POLYMORPHISM OF RANDOM AMPLIFIED
POLYMORPHIC DNA IN WHITE ROMAN GOOSE
D. Y. Lin(1) , J. J. Liu Tai (1), J. C. Chen(1) , L. T. Yeh(1) , H. L. Chang(1) and C. Tai(2)
(1)Taiwan Livestock Research Institute, Council of
Agriculture
(2)Institute of Biotechnology, National Cheng Kung University
The purpose of this study was to explore the association between production performance and RAPD (random amplified polymorphic DNA) polymorphism of White Roman Goose. Blood samples of 48 geese were collected based on mature body weight(male) and egg production performance(female). Twelve best or worst geese were assorted into each low body weight(LW), high body weight(HW), low egg production(LE) and high egg production(HE) groups. Individual genomic DNA was extracted from blood sample and detected concentration for DNA template. There were 328 kinds of a single, short (10 bp) oligonucleotide for RAPD tests. After electrophoresis in 1.5﹪ agarose gels and stained with ethidium bromide, prints of the PCR amplified products were scanned and analyzed by Gelcompar software. The results showed 85% of these primers got PCR products, and 41% got polymorphic PCR products. The primers, AC09(890bp), AE03(690bp), AE06(920bp), AG10(1350bp, 1370bp) and AL13(1288bp), produced distinct PCR products for different genders and polymorphic patterns of AD06(720bp), AD14(700bp), AH13(2400bp) and AJ15(510bp) were not independent between high and low egg production groups.
Key Words: White Roman Goose, Random amplified polymorphic DNA (RAPD), Polymorphism.