母豬造骨蛋白交替基因對產仔性能之影響

廖仁寶 張秀鑾 賴永裕 劉錦條 吳明哲

行政院農業委員會畜產試驗所

以造骨蛋白基因(OPN)微衛星型遺傳標記檢測180頭經產種母豬啟動子區域之[TG]序列重複數交替基因,其交替基因類型依所具[TG]雙核姣蘊娃じが洃嚏A計發現九種交替基因,28種基因型。在不同品種的分布分別為:於51頭藍瑞斯種母豬中,有[TG]13、[TG]14、[TG]19和 [TG]21等四種交替基因,共組成九種基因型;於54頭約克夏種母豬中,計有[TG]8、[TG]14、[TG]21、[TG]23、[TG]24和[TG]26等六種交替基因,共組成20種基因型;於25頭杜洛克豬種母豬中,計有[TG]14、[TG]21、[TG]23和[TG]24等四種交替基因,共組成六種基因型;而於50盤克夏種種母豬中,所發現的五種交替基因分別為[TG]8、[TG]13、[TG]23、[TG]24和[TG]25,共組成八種基因型。進一步分析種母豬具不同OPN交替基因對產仔性能之影響,結果顯示:具[TG]14交替基因之約克夏種母豬所分娩活仔數顯著地較未具該交替基因者為佳(8.41±0.74 vs.7.25±0.36頭,P<0.0257);具[TG]21或[TG]24交替基因之杜洛克種母豬所分娩仔豬之存活率亦顯著地較未具該交替基因者為佳(85.8±4.6 vs. 74.3±4.2%,P<0.0196;87.9±4.9 vs. 72.1±4.0%,P<0.0019);然而具[TG]13交替基因之盤克夏種母豬,其所分娩活仔數顯著地較未具該交替基因者為差(6.10±1.00 vs. 7.20±0.80頭,P<0.0495)。

關鍵語:造骨蛋白、交替基因、產仔性能。

 

INFLUENCE OF SOWS’ OSTEOPONTIN ALLELES TO THEIR LITTER PERFORMANCE

R. B. Liaw, H. L. Chang, Y. Y. Lai, C. T. Liu and M. C. Wu

Taiwan Livestock Research Institute, Council of Agriculture

A total of 180 parous sows were genotyped with osteopontin (OPN) microsatellite marker by PCR. The OPN allele was defined as consisting of different number of [TG] dinucleotide repeats in the promoter region of OPN gene. In this study, nine alleles were found and making up 28 genotypes. There were different alleles distributed among four swine breeds. In 51 Landrace pigs, four alleles including [TG]13, [TG]14, [TG]19 and [TG]21 were found and making up nine genotypes. In 54 Yorkshire pigs, six alleles including [TG]8, [TG]14, [TG]21, [TG]23, [TG]24 and [TG]26 were found and making up 20 genotypes. In 25 Duroc pigs, four alleles including [TG]14, [TG]21, [TG]23 and [TG]24 were found and making up six genotypes. In 50 Berkshire pigs, five alleles including [TG]8, [TG]13, [TG]23, [TG]24 and [TG]25 were found and making up eight genotypes. When we further analyzed the litter performance of sows with different OPN alleles, the results indicated that the number of alive piglets born from Yorkshire sows with [TG]14 allele were significantly more than those without such an allele(8.41±0.74 vs.7.25±0.36, P<0.0257). For Duroc sows, the survival rate of piglet born from sows with [TG]21 or [TG]24 allele was significantly higher than those without [TG]21 or [TG]24 allele(85.8±4.6 vs. 74.3±4.2%, P<0.0196; 87.9±4.9 vs. 72.1±4.0%, P<0.0019). But for Berkshire sows, the number of alive piglets born from sows with [TG]13 allele were significantly less than those without [TG]13 allele(6.10±1.00 vs. 7.20±0.80, P<0.0495).

Key Words: Osteopontin, Allele, Litter performance.