½Þ²É¸¢ÅéDNA D-loop°Ï°ì¤§§Ç¦C¤ÀªR

ÃC©À·O(1) ¶À¤ì¬î(2)

(1)¦æ¬F°|¹A·~©e­û·|¯b²£¸ÕÅç©Ò (2)°ê¥ß¤¤¿³¤j¾Ç¯b²£¨t

ÂÅ·ç´µ¤Î±ö¤s½Þªº½Þ²É¸¢ÅéDNA D-loop°Ï°ì§Ç¦C¤w¸g³Q´£¥X¡A¨Ãµo²{ÂÅ·ç´µ¡B±ö¤s½Þ¤Î¤j¥Õ½Þ¤§¶¡¯S®íDNA D-loop°Ï°ì¦³®t²§¡A¦¹µ²ªGÅã¥Ü½Þ²É¸¢ÅéDNA D-loop°Ï°ìªºª[²§©Ê¨ã¦³¼ç¤O¨Ñ½Þ°¦²Ó­M½è¿ò¶Çªº¤ÀªR¥Î¡C¥»¸ÕÅç³]­p¤F¤@²Õ¥i¥H½Þ¤§°ò¦]²ÕDNA¬°¼ÒªO¡A¸g¥Ñ»E¦Xþª³sÂê¤ÏÀ³¡A¼W´Þ¥XD-loop°Ï°ì392 bp¤ù¬qªº¤Þ¤l¡A§Q¥Î¦¹²Õ¤Þ¤l¼W´Þ¥X¤£¦P«~ºØ½Þ°¦¤§D-loop°Ï°ì392 bp¤ù¬q¡A¶i¦æ§Ç¦C¤ÀªR¡Cµ²ªGµo²{¦¹¤ù¬q§Ç¦C¦@¦³16­Ó®Öþ«»Ä¦ì¸mµo¥ÍÅܲ§¡A¦Ó¦b³o16­ÓÅܲ§¤¤¦³13­ÓÅܲ§¬°¦PÃþ¸m´«¡C¶i¤@¨B­pºâ¤£¦P«~ºØ½Þ°¦¶¡¤§§Ç¦C¤Àª[«×¡Aµo²{¼Ú¬ü»P¨È¬w«~ºØ¤º½Þ°¦ªº§Ç¦C¤Àª[«×¤À§O¬°0.63 ¡Ó 0.59 %»P0.51 ¡Ó 0.27 %¡A¦Ó¼Ú¬ü»P¨È¬w«~ºØ½Þ°¦¤§¶¡ªº§Ç¦C¤Àª[«×¸û¬°1.31 ¡Ó 0.59 %¡C¦¹µ²ªG¥i¨Ñ½Þ°¦²Ó­M½è¿ò¶Çºt¤Æ¤§°Ñ¦Ò¡C

ÃöÁä»y¡G½Þ¡B²É¸¢ÅéDNA¡B§Ç¦C¤ÀªR¡C

¡@

SEQUENCE ANALYSIS OF D-LOOP REGION IN THE
MITOCHONDRIAL DNA OF PIGS

N. T. Yen(1,2) and M. C. Huang(1)

(1)National Chung-Hsing University (2)Taiwan Livestock Research Institute, Council of Agriculture

The entire mitochondrial DNA (mtDNA) displacement loops (D-loops) of Landrace and Meishan have been sequenced, and the divergences of specific D-loop region among Landrace, Large White, and Meishan have been compared. The results hinted that D-loop polymorphism and/or sequence diversity is potential for molecular analysis of cytoplasmic genetic in pigs. A pair of primer was designed for the amplification by PCR to generate a 392 bp fragment of the D-loop region from the template DNA of pure pig breeds. These 392 bp PCR products were verified by their sequences. The results showed that there were 16 site substitutions in the 392 bp fragment of D-loop region. Thirteen nucleotide variations were transition. The average sequence diversities of D-loop region of the European-American and Asian breed pigs were 0.63¡Ó0.59% and 0.51¡Ó0.27%, respectively. The average sequence diversity of D-loop region between the European-American breed and Asian breed pigs was 1.31¡Ó0.59%. It showed that the sequence diversity of D-loop region is useful for evolution analysis of cytoplasmic genetic in pigs.

Key Words : Pig, mtDNA, Sequencing.