豬粒腺體DNA D-loop區域之序列分析

顏念慈(1) 黃木秋(2)

(1)行政院農業委員會畜產試驗所 (2)國立中興大學畜產系

藍瑞斯及梅山豬的豬粒腺體DNA D-loop區域序列已經被提出,並發現藍瑞斯、梅山豬及大白豬之間特殊DNA D-loop區域有差異,此結果顯示豬粒腺體DNA D-loop區域的歧異性具有潛力供豬隻細胞質遺傳的分析用。本試驗設計了一組可以豬之基因組DNA為模板,經由聚合玟s鎖反應,增殖出D-loop區域392 bp片段的引子,利用此組引子增殖出不同品種豬隻之D-loop區域392 bp片段,進行序列分析。結果發現此片段序列共有16個核姣藻鼽m發生變異,而在這16個變異中有13個變異為同類置換。進一步計算不同品種豬隻間之序列分歧度,發現歐美與亞洲品種內豬隻的序列分歧度分別為0.63 ± 0.59 %與0.51 ± 0.27 %,而歐美與亞洲品種豬隻之間的序列分歧度較為1.31 ± 0.59 %。此結果可供豬隻細胞質遺傳演化之參考。

關鍵語:豬、粒腺體DNA、序列分析。

 

SEQUENCE ANALYSIS OF D-LOOP REGION IN THE
MITOCHONDRIAL DNA OF PIGS

N. T. Yen(1,2) and M. C. Huang(1)

(1)National Chung-Hsing University (2)Taiwan Livestock Research Institute, Council of Agriculture

The entire mitochondrial DNA (mtDNA) displacement loops (D-loops) of Landrace and Meishan have been sequenced, and the divergences of specific D-loop region among Landrace, Large White, and Meishan have been compared. The results hinted that D-loop polymorphism and/or sequence diversity is potential for molecular analysis of cytoplasmic genetic in pigs. A pair of primer was designed for the amplification by PCR to generate a 392 bp fragment of the D-loop region from the template DNA of pure pig breeds. These 392 bp PCR products were verified by their sequences. The results showed that there were 16 site substitutions in the 392 bp fragment of D-loop region. Thirteen nucleotide variations were transition. The average sequence diversities of D-loop region of the European-American and Asian breed pigs were 0.63±0.59% and 0.51±0.27%, respectively. The average sequence diversity of D-loop region between the European-American breed and Asian breed pigs was 1.31±0.59%. It showed that the sequence diversity of D-loop region is useful for evolution analysis of cytoplasmic genetic in pigs.

Key Words : Pig, mtDNA, Sequencing.