台灣種豬動情素接受器多產基因頻率

陳佳萱 廖仁寶 張秀鑾

行政院農業委員會畜產試驗所

豬隻動情素接受器(Estrogen Receptor, ESR)基因座位於第一對染色體短臂上,具有兩個對偶基因A和B。文獻報告指出,ESR基因型為BB之母豬較AA型者,每胎分別可多分娩1.5與1.0頭以上仔豬與活仔豬,故亦將ESR基因座之B對偶基因稱為多產基因。本試驗應用單點核苷酸突變點拆離式聚合玟s鎖反應(MS-PCR)技術,針對台灣地區八家民間種豬場與五個試驗研究單位之種豬,進行ESR基因型逢機檢測。計完成7657頭種豬檢測,包括藍瑞斯、約克夏、杜洛克(D)、梅山豬(M)、桃園豬、盤克夏、蘭嶼豬、花色豬、迷彩豬與畜試黑豬一號,以及M與D之雜交豬。結果發現:除M與D之雜交豬外,前述各品種ESR基因型為BB型之頻率分別為0.3%、26.6%、0%、100%、80.0%、1.0%、16.7%、10.8%、31.0%與1.9%;B對偶基因頻率則分別為0.05、0.51、0.00、1.00、0.81、0.01、0.42、0.46、0.50與0.16。

關鍵詞:動情素接受器、基因頻率、豬隻。

 

THE PROLIFIC GENE FREQUENCY OF ESTROGEN
RECEPTOR LOCUS FOR BREEDING PIGS IN TAIWAN

C. H. Chen, R. B. Liaw and H. L. Chang

Taiwan Livestock Research Institute, Council of Agriculture

The estrogen receptor (ESR) locus in swine was mapped at p-arm of chromosome one with two alleles, A and B. Literature indicated that BB homozygotic sows produced 1.0 and 1.5 more piglets at birth and born alive, respectively than did AA homozygotes. And thus, B allele of ESR locus is generally recognized as prolific gene. Mutagenically separated polymerase chain reaction (MS-PCR) technique was applied in the study for ESR genotyping. A total of 7657 breeding pigs from eight private breeding farms and five research stations were blood sampled for genotyping. The genotypic frequencies of BB homozygotes were 0.3%, 26.6%, 0%, 100%, 80.0%, 1.0%, 16.7%, 10.8%, 31.0% and 1.9% for Landrace, Yorkshire, Duroc, Meishan, Taoyuan, Berkshire, Lanyu, Spotty Lanyu, Mitsae and TLRI-Black, respectively. The corresponding gene frequencies for prolific gene B allele were 0.05, 0.51, 0.00, 1.00, 0.81, 0.01, 0.42, 0.46, 0.50 and 0.16, respectively.

Key Words: Estrogen receptor, Gene frequency, Swine.