台灣商業豬種動情素受體基因頻率分析

陳佳萱(1) 賴永裕(1) 劉桂柱(2) 張秀鑾(1)

(1)行政院農業委員會畜產試驗所 (2)台灣區種豬發展協會

本試驗針對90-91年台灣地區商業用種豬(L、Y、D)進行動情素受體基因座逢機檢測，共蒐集19家種豬場，2,102頭種公母豬，包括藍瑞斯723頭、約克夏120頭、杜洛克1259頭。文獻指出，豬隻動情素受體(Estrogen receptor, ESR)基因座位於豬第一對染色體短臂上，具有兩個交替基因A和B，ESR基因型為BB之母豬較AA型者，每胎分別可多分娩1.5與1.0頭以上仔豬與活仔豬，故亦將ESR基因座之B交替基因稱為多產基因。本試驗檢測方式採限制脢分切片段長度多態性(RFLP)與單點核苷酸突變點拆離式聚合脢連鎖反應(MS-PCR)方式兩者交叉比對，減少判讀錯誤率。基因頻率檢測結果顯示，藍瑞斯B交替基因頻率為0.07、約克夏0.28、杜洛克則為0.01。

關鍵語：動情素受體、基因頻率、豬隻。

THE PROLIFIC GENE FREQUENCY OF ESTROGEN RECEPTOR IN
TAIWAN COMMERCIAL BREEDING STOCKS

C. H. Chen(1), Y. Y. Lai(1),,K. C. Liu(2) and H. L. Chang(1)

(1)Taiwan Livestock Research Institute of COA (2)Formasan Farmers Association for Swine Improvement

To understand gene frequencies of estrogen receptor Locus of commercial pure breeds, 2102 sows, including 723 Landrances, 120 Yorkshires and 1259 Durocs, were blood sampled from 19 breeding farms in year 2001 and 2002. The estrogen receptor (ESR) locus in swine was mapped at p-arm of chromosome one with two alleles, A and B. Literature indicated that BB homozygotic sows produced 1.0 and 1.5 more piglets at birth and born alive, respectively than did AA homozygotes. And thus, B allele of ESR locus is generally recognized as prolific gene. Both mutagenically separated polymerase chain reaction (MS-PCR) technique tested and restriction fragment length polymorphism (RFLP) analysis were used to test each sample to increase the accuracy of results. The gene frequencies for prolific gene B allele were 0.07 for Landrance, 0.28 for Yorkshire and 0.010 for Duroc, respectively.

Key Words: Estrogen receptor, Gene frequency, Swine.