羊黏多醣症遺傳缺陷之DNA檢測

林德育 黃鈺嘉 魯學智 張秀鑾

行政院農業委員會畜產試驗所

山羊黏多醣症 (Mucopolysaccharidosis) 第三型，俗稱G-6-S，是一種遺傳缺陷所導致的代謝性疾病，且為一簡單的隱性基因遺傳。為初步瞭解該遺傳疾病在台灣羊隻的概況，以1998採集東部某一羊場的努比亞山羊種羊20頭(2公18母)、吐根堡山羊20頭及台灣土山羊21頭血樣DNA，共計61個樣品進行檢測，初步結果在努比亞山羊中高達25%(5/20)帶有此不良基因(雜合型)，而在所有檢測的吐根堡山羊與台灣土山羊皆為正常型。再採集該場2002年8月全場141頭努比亞山羊進行檢測，有15%(21/141)羊隻為雜合型個體，無有病型(隱性純合型)個體，顯示該不良基因存在於該羊場中之努比亞山羊族群中。由於努比亞山羊在台灣肉用山羊生產體系中扮演重要角色，了解此一不良基因在台灣羊群之頻率有其需要性。

關鍵語：山羊、頻率、黏多醣症。

DNA TYPING OF INHERITED DEFICIENCY OF CAPRINE
MUCOPOLYSACCHARIDOSIS IIID

D. Y. Lin, Y. C. Huang, G. Lu and H. L. Chang

Taiwan Livestock Research Institute, Council of Agriculture

Caprine Mucopolysaccharidosis IIID, or N-acetylglucosamine 6-sulfatase deficiency (G6S), is a recessive inherited disorder in goat. To explore the impact of G6S on goat industry, a total of 61 samples collected from private goat farm of eastern Taiwan in 1998, were tested. Samples represented Nubian (18 females and 2 males), Toggenburg (16 females and 4 males) and Taiwan native goat (20 females and 1 male). Five carrier goats were found in 20 Nubian with frequency being 25%. No carrier was found in Toggenburg or Taiwan native goat. Further screen were performed by 141 blood DNA samples from all Nubian goats’ of the farm in Aug. 2002. The frequency of carried was 15%(21/141) which explores risk of high frequency of G-6-S of Taiwan Nubians existed. No affected individual, homozygous recessive goat, was found in this trial. Nubian is one of the major resource goat breeds for meat production in Taiwan, and thus the corresponding frequency of G6S deserves to gain more attention.

Key Words: Goat, Frequency, Mucopolysaccharidosis.