135

39.雞種的熱休克蛋白70基因頻率

林德育　鍾秀枝　黃鈺嘉　黃祥吉　林宗貴　吳明哲

行政院農業委員會畜產試驗所

熱休克蛋白(Heat shock protein, HSP)是指細胞在反應刺激原的誘導下所生成的一組蛋白質，特別是環境高溫的誘導。熱休克蛋白70 (HSP70) 是熱休克蛋白家族的一重要成員，在動物細胞生長、發育、繁殖、基因表現及蛋白質合成扮演重要的角色。雞隻受熱緊迫時HSP70在許多組織有高的活性表現，此現象可能與已知熱休克蛋白70的多態性有關。從84隻來亨雞、48隻白肉雞、104隻紅羽土雞、以及590隻畜試土雞近親品系L7、L9、L11與L12：分別有99、174、155及162隻血樣，萃取DNA，並應用聚合酶連鎖反應與單股構形多態性(Single Strand Conformation Polymorphism, SSCP)方法來檢測HSP70基因型。總共826個樣品，檢出3種基因型 (AA、AB及BB)。因此，HSP70的交替基因有A 與 B兩個。HSP70的交替基因A 頻率在來亨蛋雞、白肉雞、紅羽土雞、畜試土雞近親品系L7、L9、L11及L12分別為0.03、0.09、0.37、0.93、0.59、0.62及0.63。本土雞種畜試土雞近親品系及紅羽土雞的交替基因HSP70A 頻率顯著地高於外來雞種來亨蛋雞及白肉雞基因頻率(P<0.001)，本土雞種的HSP70A基因頻率高，外來雞種的HSP70B基因頻率高。

關鍵語：雞、熱休克蛋白70、單股構形多態性

ALLELE FREQUENCY OF HEAT SHOCK PROTEIN 70 GENE IN CHICKENS

D. Y. Lin, H. C. Chung, Y. C. Huang, H. C. Huang, J. C. Lin, and M. C. Wu

Livestock Research Institute, Council of Agriculture

Heat shock protein 70 (HSP70) is an important member of heat shock protein family and play an important role in animal cell growth, development, and prolificacy, gene expression and protein synthesis, folding and transportation. HSP70 is induced by heat stress and highly expresses in many tissues under heat stress. Genomic DNA samples were extracted from blood samples of 84 white Leghorn layers, 48 white commercial broilers, 104 red feather native chickens, and a total of 590 birds from four inbreed lines of TLRI native chicken. Blood samples of TLRI inbred lines were 99 of L7, 174 of L9, 155 of L11 and 162 of L12. All of 826 samples were used in the PCR-SSCP method to detect HSP70 genotype. Three genotypes of AA, AB and BB were classified. The gene frequency of allele A of HSP70 locus were 0.03 in white Leghorn layer, 0.09 in white commercial broiler, 0.37 in red feather native chicken, 0.93 in L7, 0.59 in L9, 0.62 in L11, and 0.63 in L12, respectively. Results indicated that native breeds of TLRI inbred lines and red feather native chicken had a significant higher frequency of HSP70A allele than exotic breeds of Leghorn layer and white broiler had (P<0.001). Native chicken has a higher frequency of HSP70A allele and exotic breeds had more HSP70B allele.

Key Words: Chicken, Heat shock protein 70, SSCP