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44.蘭嶼豬粒線體細胞色素氧化酶第一次單元基因全長定序

陳佳萱^{(1) (3)} 楊绣雅⁽¹⁾ 顏念慈⁽¹⁾朱賢斌⁽²⁾ 陳坤照⁽²⁾ 吳明哲⁽¹⁾ 黃木秋⁽³⁾

⁽¹⁾行政院農業委員會畜產試驗所 ⁽²⁾行政院農業委員會畜產試驗所台東種畜繁殖場 ⁽³⁾國立中興大學動物科學系

粒線體DNA是一雙股構成的環狀分子，哺乳動物大約為15-17 Kb，胞器組成包括2個核糖體核醣核酸 （ribosomal RNA, rRNA），22個轉譯核糖核酸（transfer RNA, tRNA），13個蛋白質基因 （protein coding genes），與一段D環區 （control region, D-loop）。粒線體細胞色素氧化酶第一次單元序列分析 (cytochrome c oxidase subunit 1, CO1)已被列入物種基因條碼的重要標示區域，本試驗設計兩對引子成功完成蘭嶼豬CO1基因核苷酸序列，全長共1545 bp，以blast軟體與其他序列比較，發現蘭嶼豬CO1基因與其他種豬間序列相似度高達99％，與其他物種序列歧異度分別為牛81％、山羊82％、梅花鹿81％、馬81％與雞77％。成功完成粒線體CO1基因有助畜產應用在基因條碼編列與物種鑑定。

關鍵語：細胞色素氧化酶第一次單元、粒線體DNA、蘭嶼豬

PRIMARY ANALYSIS OF MITOCHONDRIAL DNA CYTOCHROME C OXIDASE SUBUNIT I REGION SEQUENCE IN LANYU PIG

C. H. Chen^{(1) (3)}, H. Y. Yang⁽¹⁾,  N. T. Yen⁽¹⁾, H. P. Chu⁽²⁾, K. J. Chen ⁽²⁾, M. C. Wu⁽¹⁾ and M. C. Huang⁽³⁾

⁽¹⁾Livestock Research Institute, Council of Agriculture, Executive Yuan

⁽²⁾ Taitung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Executive Yuan

 ⁽³⁾Department of Animal Science, National Chung- Hsing University

Mitochondrial DNA is a circular structure of double strain nucleotide, about 15-17 Kb in mammals. There are 2 ribosomal RNA, 22 transfer RNA, 13 protein coding gene and 1 D-loop region in mitochondrion. Mitochondrial cytochrome c oxidase subunit I (CO1) has become an important marker region for species barcode. Two pair of primers, a total length of 1545 bp, were designed in this trial to sequence CO1 nucleotide in Lanyu pig. Comparing with the sequence of other species by blast software, the similarity of CO1 between Lanyu and other pig breeds reached a high level of 99 %; while the divergence among Lanyu and other species were 81 % in cow, 82 % in goat, 81 % in Sika deer, 81 % in horse and 77 % in chicken. The results implicated that mitochondrial CO1 was applicable for barcode editing and species identity in farm animals.

Key Word：Cytochrome c oxidase subunit 1, Mitochondrial DNA, Lanyu pig