中國畜牧學會會誌35(增刊)  ,2006
J. Chin. Soc. Anim. Sci. 35(Suppl.)

161

97.種豬飼料效率遺傳標記選拔族群建立

 

顏念慈(1)、陳裕琪(1)、 楊琇雅(1)、 朱賢斌(1)、 陳佳萱(1)、 張秀鑾(2)、 陳坤照(1)、吳明哲(1)

行政院農業委員會畜產試驗所(1) 國立屏東科技大學(2)

 

台灣豬隻飼料原料大多仰賴進口,故長期以來豬隻飼料效率被列為種豬群生長性能選拔的重要性狀之一。遺傳標記輔助選拔技術的應用,可加速豬飼料效率性狀之改良。選留中央檢定站北站92-2期飼料效率第一名之藍瑞斯公豬,藍瑞斯女豬選留具飼料效率多樣性之瞼豬群;飼料效率較差之賓朗豬則選留一公及四母為飼料效遺傳標記選拔豬。源自中央檢定站瑞斯公豬L727-11飼料效率為1.48,試驗場之藍瑞斯族群飼料效率平均為2.7,選留藍瑞斯女豬飼料效率分布範圍介於2.26~3.22間,而賓朗豬平均飼料效率為3.6。所有參試種公與母豬之緊迫基因皆為CC正常型、多產基因僅少數為B產型,而肉質基因之組點基因型則為LL2,HL3與HL4基因型。此外,進行50組分布於各染色體之微衛星型遺傳標記的檢測;基定位出豬飼料效率基因,並進一開發相關重要基因標記供豬種改良用。

關鍵語:飼料效率、遺傳標記、豬

 

CONSTRUCTION OF REFERENCE POPULATION FOR MAPPING FEED EFFICIENCY RELATED GENE(S) IN PIG

In taiwan, most of feed ingredients for pig production are imported and thus feed efficiency (FE) is one of the most important traits in swine selection program.   The marker-asisted selection (MAS) not only provides an opportunity but also accelerates the genetic progress of FE in pig production.  To map the feed efficiency related gene(s), Landrace and Peinan breeds were used to establish the reference population.  Landrace boar, ear notch being 727-11, with the best feed efficiency (FE=1.48) among pigs tested in the 92-2 term at the north central test station was selected and served as founder sire.  Furthermore, the average and range of FE in Landrace gilts used in founder population were 2.7 and 2.26~3.22, respectively.  for reciprocal cross purpose, one Peinan boar and for Peinan gilts were used in the study.  Peinan pigs had poor FE as usual with the average FE being 3.6.  All the founder animals were stress free (CC type), but only few pigs with BB genotype was observed when estrogen receptor was genotyped.  The combined genotypes of heart fatty acid binding protein found in founders were LL2, HL3 and HL4.  In addition, fifty microsatellites spread around 19 pairs of chromosomes were also screened for FE related gene mapping and exploration of the important flunking marker(s) for breeding program.

Key Words: Feed efficiency, Genetic marker, Swine