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92.建立台灣梅花鹿粒線體基因條碼

陳佳萱(1) (3) 姜力嘉(3) 張慕慈(3) 康獻仁(2) 王治華(2) 吳明哲(1) 黃木秋(3)

(1)行政院農業委員會畜產試驗所 (2)行政院農業委員會畜產試驗所高雄種畜繁殖場 (3)國立中興大學動物科學系

粒線體用來建立動植物基因條碼是熱門研究，以序列歧異度建立基因條碼是新興分類方法，全世界仍有許多物種無法被分類，利用粒線體細胞色素C氧化酶I（cytochrome c oxidase subunit 1，CO1）區域，可做為一種快速、便宜、且可信的物種鑑別方法，此法雖然遭受分類學家質疑，但卻無法壓抑這股熱門研究風潮。本試驗設計5對引子，可增殖台灣梅花鹿（Cervus nippon taiouanus）粒線體tRNA-Tyr、CO1、tRNA-Ser、tRNA-Thr、tRNA-Pro與D-loop基因，序列總長度2,818 bp，經由定序多個基因序列，可以增加基因條碼鑑識物種正確性與可信度，減少錯誤判斷率，並做為畜產種原與生產履歷發展之用。

關鍵語：粒線體、台灣梅花鹿、基因條碼

SET UP THE MITOCHONDRIAL DNA BARCODE IN TAIWAN FORMOSA DEER

C. H. Chen(1) (3), L. C. Chiang(3), M. C. Chang(3) , H. J. Kang (2), C. H. Wang(2), M. C. Wu (1), and M. C. Huang(3)

(1)Livestock Research Institute, Council of Agriculture, Executive Yuan

(2) Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Executive Yuan

 (3)Department of Animal Science, National Chung Hsing University

It is a hot research work which utilizing sequence diversity degree of mitochondrial DNA to set up the DNA barcode in the animals and plants. There are many species cannot be classified in the world yet, but examine of mitochondrial DNA cytochrome c oxidase subunit 1 (CO1) area offers a kind of fast, cheap and creditable method to classify species differentiation. Although this way is queried by taxonomist, this hot research tendency has widely spread. Five pairs of primers having 2,818 bp were designed to amplified the fragments of tRNA-Tyr, CO1, tRNA-Ser, tRNA-Thr, tRNA-Pro genes and D-loop region in Formosan sika deer mitochondrial DNA. By sequencing a lot of gene sequences, the DNA barcodes of mitochondrial DNA not only increase the accuracy and credibility as well as reduce the mistaken judgment among species distinction but also apply to the animal genetic resources and production traceability.

Key Words: Mitochondrial DNA, Formosan sika deer, DNA barcode