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64.畜產種原庫之自動冷凍記錄平台建置

賴永裕 林德育 蕭庭訓 黃鈺嘉 吳明哲

行政院農業委員會畜產試驗所

台灣畜產種原中心於2004年建好，座落於台南新化，建築物主要包括生殖細胞冷凍保存庫、DNA冷凍保存庫與種原資訊庫等三部份。國家級設備完善的超低溫冷凍保存種原庫，需具有自動化低溫監控設備，經由儀器操控，在儲藏庫外面便能輕易進行遠端種源品項的保存歷程記錄，以維持遺傳物質長期冷凍品質。本研究整合畜產種原庫超低溫冷凍保存用的18個大型液氮儲存槽之電子控制器，在不影響原有操作狀態均連線到中央監控室的電腦成遠端電腦控制記錄系統，使電腦上可同步顯示每個液氮儲存槽液位高低值、溫度值，建立遺傳物質冷凍品質之自動監控標準操作及遠端預警系統。並可由電腦端即時下載儲存槽控制器內的溫度記錄、液位高低值成格式檔供彙整進入畜產種原資訊資料庫，2008年5月至10月間共記錄72,260筆紀錄，11筆系統異常警示紀錄，桶槽最高溫度與最低溫度在-172℃與-182℃，正常液位最低3英吋，最高5英吋，不正常液位7筆皆為電磁閥故障造成液氮過度充填。結果顯示，自動化低溫監控記錄系統可用於冷凍保存品質的提昇，而長程種原遺傳物質的妥善保存即是保護國家最珍貴的天然寶藏。

關鍵語：液氮、冷凍系統、種原

A TRAIL OF AUTO-RECORDING SYSTEM FOR LIQUID NITROGEN CRYOSYSTEM OF ANIMAL GENETIC RESOURCES

Y. Y. Lai, D. Y. Lin, T. H. Hsiao, Y. C. Huang and M. C. Wu

Livestock Research Institute, Council of Agriculture

Construction of Taiwan Animal Germplasm Center was completed in 2004 at Hsinhua, Tainan and three main sections of the center are germ cell bank, DNA bank and data bank of genetic resources.  For quality control, remote monitoring system was essential for the long term cryopreservation.  This trail set up an integrated electronic monitoring system for 18 large liquid nitrogen(LN₂) tank, including temperature and liquid position of LN₂ recording, and remote auto-alarming.  The monitoring data have been cumulated into animal genetic resource databank for study.  From May to Oct. 2008, databank added into 72,260 records.  The range of maximum and minimum temperature was from -172℃ to -182℃.  The range of liquid position of LN₂ was from three to five inches. There were 11 alarming messages received.  Seven records of abnormal liquid positions of tank were due to solenoid valve errors, which caused LN₂ overfilling in.  Results showed auto-recording system can be used for improve cryopreservation quality and the success of long-term genetic resources conservation program will protect the most valuable nature treasure of Taiwan.

Key words: Liquid nitrogen, Cryosystem, Genetic resource