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96.畜試土雞微衛星遺傳標記多樣性分析

林德育  黃文瑛  黃鈺嘉  賴永裕  廖仁寶  林義福  吳明哲

行政院農業委員會畜產試驗所

為建立台灣土雞遺傳標記與基因條碼資訊。本試驗利用10組雞微衞星標記組，分析行政院農業委員會畜產試驗所畜試土雞96隻種雞個體DNA，所有微衞星標記皆有多態型的基因型。共檢測到76個alleles，平均每個基因座具有7.6個對偶基因(6~9個alleles)，其觀測異質度介於0.302到0.990，平均為0.653 ± 0.236，期望異質度介於0.360到0.849，平均為0.716 ± 0.157，而多態性訊息含量介於0.339到0.828，平均為0.684 ± 0.162。有7組雞微衞星標記組(70%)具高多態性訊息含量(PIC>0.5)，而其餘3組(30%)為合理的多態性訊息含量(0.5>PIC>0.25)，顯示本試驗選用之微衞星標記組具豐富的多態性訊息，可作為土雞遺傳多樣性監控與個體基因條碼識別之遺傳標記。

關鍵語：土雞、遺傳多樣性、微衞星標記

GENETIC DIVERSITY ANALYSIS OF NATIVE CHICKEN BY MICROSATELLITE MARKERS

D. Y. Lin, W. Y. Huang, Y. C. Huang, Y. Y. Lai, R. B. Liaw, Y. F. Lin and M. C. Wu

Livestock Research Institute(LRI), Council of Agriculture

In order to establish chicken genetic marker and DNA barcode information, ten  microsatellite markers were utilized for generating microsatellite genotyping data in a panel of 96 breeding native chicken of Livestock Research Institute(LRI).  All the microsatellites were highly polymorphic, with mean (± SD) allelic number of 7.6 ± 1.22 and ranged from 6 to 9 alleles per locus. The average observed heterozygosity in the population ranged between 0.302 and 0.990, with mean (± SD) of 0.653 ± 0.236.  The expected heterozygosity ranged between 0.360 and 0.849, with mean (± SD) of 0.716 ± 0.157.  The polymorphic information content (PIC) was estimated with mean (± SD) of 0.684 ± 0.162 and ranged between 0.339 and 0.828.  Seven microsatellite  markers(70%) were highly informative (PIC>0.5) and the other three markers(30%) were reasonably informative (0.5>PIC>0.25).  The result indicated these ten markers could be used for monitoring genetic diversity and making the DNA barcodes for LRI native chicken.

Key Words: Chicken, Genetic diversity, Microsatellite marker