中國畜牧學會會誌38(增刊)

 ,2009

J. Chin. Soc. Anim. Sci. 38(Suppl.)

131

51.台灣本地黑山羊微衛星遺傳標記多樣性分析

 

林德育 楊深玄 賴永裕 李光復 黃政齊 吳明哲

行政院農業委員會畜產試驗所

 

台灣本地黑山羊是台灣重要的本土畜產動物,自1987年起設立國家保種場於行政院農業委員會畜產試驗所甯K分所,至今台灣本地黑山羊遺傳多樣性資料仍然有限。本試驗利用FAO(2004)建議使用的山羊微衛星標記組,以其中12組山羊微衛星標記分析行政院農業委員會畜產試驗所甯K分所台灣本地黑山羊保種族群40頭山羊個體之DNA,其中除ETH10微衛星標記所檢測的基因型在所有檢測個體皆為單型外,其它11組微衛星標記皆有多態型的基因型。具多態型的微衛星標記組共檢測到42個alleles,平均每個基因座具有3.8個對偶基因(2~6個alleles),其觀測異質度介於0.262到0.714,平均為0.483,期望異質度介於0.357到0.674,平均為0.502,而多態性訊息含量平均為0.434。在本試驗選用的12組微衛星標記組中有10組呈現非高多態性資訊(PIC<0.5),且有1組微衛星標記(ETH10)在所有檢測樣品僅出單一等位基因型,顯示育種者應更加重視台灣本地黑山羊遺傳多樣性的維護。

 

關鍵語:台灣本地黑山羊、遺傳多樣性、微衞星標記 

 

 

GENETIC DIVERSITY ANALYSIS OF TAIWAN BLACK GOAT BY MICROSATELLITE MARKERS

 

D.Y. Lin, S.S. Yan, Y.Y. Lai, J.C. Huang and M.C. Wu

Livestock Research Institute(LRI), Council of Agriculture

 

Taiwan Black Goat is an important indigenous farm animal in Formosa Island. The conservation population was in Hengchung Branch of LRI since 1987. However, the information of genetic diversity of Taiwan Black Goat was very limited. To study the genetic diversity, a set of 12 microsatellite markers recommended for goat in FAO’s DADIS MoDAD programme were utilized. The study was carried out on 40 animals to generate genotype data. Except ETH10, all the microsatellites were polymorphic with average allelic number 3.8, ranged from 2 to 6 per locus. There were 42 alleles detected in total. The observed heterozygosity of the population ranged from 0.262 to 0.714, and the average observed heterozygosity was 0.483. The expected heterozygosity ranged from 0.357 to 0.674, and the average expected heterozygosity was 0.502. The estimated polymorphic information content (PIC) was 0.434. In 12 markers, ten markers were not highly informative with PIC less than 0.5 and one marker ETH10 had only one allele detected. Therefore, geneticist should be aware of genetic diversity of Taiwan Black Goat.

 

Key Words: Taiwan Black Goat, Genetic diversity, Microsatellite marker