中國畜牧學會會誌38(增刊)

 ,2009

J. Chin. Soc. Anim. Sci. 38(Suppl.)

134

54.台灣黃牛微衛星遺傳標記多樣性分析

 

林德育 李光復 賴永裕 黃政齊 吳明哲

行政院農業委員會畜產試驗所

 

台灣黃牛是台灣重要的本土畜產動物,自1987年起設立國家保種場於行政院農業委員會畜產試驗所甯K分所,至今台灣黃牛遺傳多樣性資料仍然有限。本試驗利用FAO(2004)建議使用的牛微衛星標記組,以其中12組牛微衛星標記分析行政院農業委員會畜產試驗所甯K分所台灣黃牛保種族群93頭台灣黃牛個體之DNA,所有12組微衛星標記皆有多態型的基因型。具多態型的微衛星標記組共檢測到106個alleles,平均每個基因座具有8.8個對偶基因(5~13個alleles),其觀測異質度介於0.37到0.87,平均為0.57 ± 0.15,期望異質度介於0.52到0.83,平均為0.73 ± 0.09,而多態性訊息含量平均為0.69 ± 0.10。在本試驗選用的12組微衛星標記組皆有多態型的基因型,且除BM1824微衞星標記之多態性訊息含量為中多態性資訊(0.25<PIC<0.5)外,其它11組微衞星標記之多態性訊息含量均為具高多態性資訊(PIC<0.5),顯示育種者對台灣黃牛遺傳多樣性的維護與重視。

 

 關鍵語:台灣黃牛、遺傳多樣性、微衞星標記 

 

 

GENETIC DIVERSITY ANALYSIS OF TAIWAN YELLOW CATTLE BY MICROSATELLITE MARKERS

 

D.Y. Lin, G.F. Li, Y.Y. Lai, J.C. Huang and M.C. Wu

Livestock Research Institute(LRI), Council of Agriculture

 

Taiwan Yellow Cattle is an important indigenous farm animal in Formosa Island. The conservation population was in Hengchung Branch of LRI since 1987. However, the information of genetic diversity of Taiwan Yellow Cattle was very limited. To study the genetic diversity, a set of 12 microsatellite markers recommended for cattle in FAO’s DADIS MoDAD programme were utilized. The study was carried out on 89 animals to generate genotype data. All of the microsatellites were polymorphic with average allelic number 8.8, ranged from 5 to 13 per locus. There were 106 alleles detected in total. The observed heterozygosity of the population ranged from 0.37 to 0.87, and the average observed heterozygosity was 0.571±0.15(mean±SD). The expected heterozygosity ranged from 0.52 to 0.83, and the average expected heterozygosity was 0.73±0.09. The estimated average polymorphic information content (PIC) was 0.69± 010. Except BM1824 was reasonably informative (0.50 > PIC > 0.25), all the microsatellites were highly informative highly informative (PIC > 0.50). Our result indicated that the geneticists have paid more attention in keeping genetic diversity of the Taiwan Yellow Cattle herd.

 

Key Words: Taiwan Yellow Cattle, Genetic diversity, Microsatellite marker