J. Chin. Soc. Anim. Sci. 39(Suppl.)
廖仁寶 陳若菁 林秀蓮 康獻仁 王治華 程梅萍 吳明哲
應用鹿隻mtDNA cytb基因與D-loop區專一之引子對，進行32個水鹿樣品DNA之PCR增幅放大，純化產物後，進行序列解析，將最後所得之各30條序列分別建構類源關係演化圖，發現若以cytb基因序列分析時，水鹿族群可分成主要的兩群，一群由27條序列組成，另一群則由3條序列組成，但若以D-loop區序列進行比對分析時，則可得知水鹿族群可分成五群，由此顯示D-loop區序列的演化變異率較cytb基因為大。此外，以16組微衛星型遺傳標記分析32頭台灣水鹿之基因多樣性，發現其觀測異質度、期望異質度及多態性訊息量範圍分別為0–0.84、0–0.73及0–0.68，而其平均值則分別為0.32± 0.24、0.38 ±0.27及0.34 ± 0.25，可得知此群水鹿的基因多樣性屬於低度多態性資訊。
PHYLOGENETIC AND GENETIC DIVERSITY ANALYSES OF SAMBAR DEER
R. B. Liaw, R. J. Chern, H. L. Lin, S. R. Kang, C. H. Wang, M. P. Cheng and M. C. Wu
Livestock Research Institute, Council of Agriculture, Executive Yuan
The primer sets specific to cytb gene and mitochondrial D-loop region of deer were designed and used to amplify 32 sambar deer DNA. After purification, the PCR products were sequenced by an automatic DNA sequencer. The sequences of cytb gene and D-loop region from 30 samples were used to construct phylogenetic trees, respectively. Based on cytb gene analysis, the phylogenetic tree had two clusters; one cluster was composed of 27 sequences, the other 3 sequences. Based on the mitochondrial D-loop sequence analysis, the 30 sequences were divided into five clusters. The result indicates that the evolution rate of D-loop region is faster than that of cytb gene. Besides, the genetic diversity analysis of 32 sambar deer was conducted by using 16 microsatellite markers. The values for observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) among all loci were in the range of 0–0.84、0–0.73 and 0–0.68, respectively. Furthermore, the mean values for Ho, He, and PIC were 0.32±0.24、0.38±0.27 and 0.34±0.25, respectively. The result indicates that the genetic diversity of this sambar deer population is low.
Key Words: Sambar deer, Phylogenetic tree, Genetic diversity.