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88.黑山羊基因多樣性分析

廖仁寶 陳若菁 林秀蓮 莊璧華 林正鏞 程梅萍 吳明哲

行政院農業委員會畜產試驗所

本研究之目的在於利用微衛星型遺傳標記探討黑山羊之基因多樣性。以9組微衛星型遺傳標記分析60頭行畜產試驗所花蓮種畜場之黑山羊DNA，結果顯示標記之交替基因數目介於2–9個，平均交替基因數為6.22±2.11，而觀測異質度(Ho)、期望異質度(He)及多態性資訊含量(PIC)則分別為0.53±0.02、0.59±0.06及0.55±0.18。在ETH10標記的分析中發現，所有山羊個體的基因型僅兩種，分別為198/198（10%）與208/208（90%），故Ho之數值為零，而其他8種標記分析所得之Ho與He幾乎不小於0.50。各標記除ETH10與ILSTS005外之PIC，均大於0.5，且各標記之平均PIC值亦大於0.5，故族群具有高度遺傳多態性資訊。

關鍵詞：黑山羊、微衛星型遺傳標記、基因多樣性。

GENETIC DIVERSITY OF BLACK GOATS EVALUATING BY MICROSATELLITE MARKERS

R. B. Liaw, R. J. Chern, H. L. Lin, P. H. Chuang, J. Y. Lin M. P. Cheng and M. C. Wu

Livestock Research Institute, Council of Agriculture, Executive Yuan

The aim of this study was to investigate the genetic diversity of black goats using microsatellite genotyping. A total of nine microsatellite markers were used to genotype 60 black goats of Hualien propagation station, Livestock Research Institute. The allele number for all loci ranged between 2 to 9, and the mean allele number was 6.22±2.11. The mean values for observed heterozygosityg (Ho), expected heterozygosity (He), and polymorphism information content (PIC) were 0.53±0.02, 0.59±0.06 and 0.55±0.18, respectively. From the analysis of ETH10 marker, there were only two genotypes with 198/198（10%）and 208/208（90%）in the goat population. Therefore, Ho of this goat population was zero. The values of Ho and He deduced from other loci were almost no less than 0.5. PIC values obtained from the analyses of microsatellite markers except for ETH10 and ILSTS005 were larger than 0.5, and the mean values of all markers were also larger than 0.5, indicating that the genetic diversity of this goat population was high.

Key Words: Black goat, Microsatellite marker, Genetic diversity.