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98.種豬與種土雞之產精能力之研究

郭廷雍、林秀蓮、邢湘琳、陳佳萱、林德育、賴永裕、廖仁寶、顏念慈、吳建興、吳明哲

行政院農業委員會畜產試驗所 遺傳育種組

由於我國種豬場與種土雞場，早已使用生長性能檢定、人工授精與產精基因等DNA鑑定技術來選育後裔，未來若能將產精能力併入現代種畜禽選種之架構，將可提昇畜禽生產性能檢定體系強化及建置並且減少因選留產精不良種畜禽所造成之損失。本研究目的乃藉由精子體能分析儀針對精子六項體能測定及一項精液性狀測定，完成每頭受測公猪至少建立1000至5000隻精子體能分佈圖型，來檢定高肉質公豬與土雞之產精能力，作為未來選留產精能力強的種公豬與種土雞之應用。試驗中分別將採得之新鮮種豬及種雞精液，分別予以稀釋成0.5×106 /ml分析濃度後，依各分析項目加入所需染劑後，於37℃培養數分鐘後，上機分析該樣品之活/死精子數、穿孔體及精子膜完整性、線粒體膜電位完整性與細胞凋亡比例、細胞內游離鈣水平的檢測、DNA染色質結構完整性、精子細胞內自由基及精漿細菌污染等分析後，初步結果顯示在建立精子體能分佈圖上大致符合分析儀對該品種設定之內部參數，但在不同種豬間（藍瑞斯、約克夏與杜洛克）及種雞（紅羽土雞）精子體能分佈圖，則存在品種及個體間差異，該差異是否受到品種特異性或飼養及環境外在因子之影響，則有待進一步分析與比較之。

關鍵語：公豬、公雞、精子分析、精子品質、細胞分選儀

THE STUDY OF SPERM PRODUCTION PERFORMANCE TEST IN BREEDING PIGS AND ROOSTERS

T.Y. Kuo, H.L. Lin, H.L Hsing, D.Y. Lin, Y.Y. Lai, N.T. Yen, C.H. Chen, R.B. Liaw, C.H. Wu and M.C. Wu.

Livestock Research Institute, Council of Agriculture

In Taiwan, growth performance test, artificial insemination and reproductive gene typing are adopted as selection of progeny of breeding stocks in early of 1990s. Hence, further to establish an evaluation system on sperm capacity in high meat quality populations of breeding pig and chicken, it will be beneficial to having improvement of better prolificacy on semen production of breeding animal selection program and reduce the cost of keeping sterile male .The purpose of this study is to establish a technique for evaluation of sperm quality and evaluate at least 1000 to 5000 sperms of test breeding animals as predictors in young breeding animal for selection. First, the semen diluted to 0.5×106 /ml will be mixed with specific dye and incubate at 37℃for minutes, then analyze sperms with viability, acrosome and sperm membrane integrity, mitopotential, calcium level , chromatin structure,oxidation and bacterial count assay. The result shows that different indexes were found in different pig species and also in individual but same species. The criteria of this semen evaluation techniques may need further study.

Key words: boar, rooster, sperm analysis, sperm quality, flow cytometry