J. Chin. Soc. Anim. Sci. 39(Suppl.)
林德育(1) 林義福(1) 邢湘琳(1) 林秀蓮(1) 賴永裕(1) 張秀鑾(2) 吳明哲(1)
本試驗利用畜試土雞近親品系9與來亨雞進行正反雜交產生雜交一代雞隻，雜交一代雞隻進行全同胞配種產生雜交二代雞隻，以建立參考族群。採集參考族群雞隻血樣萃取DNA，進行動情素接受體基因(ESR)基因型檢測，並結合母雞產第1個蛋之日齡記錄進行關聯性分析。畜試土雞近親品系9 雞隻ESR基因型頻率EE、EF及FF分別為0%、3.0%及97.0%，而來亨雞則分別為61.1%、33.3%及5.6%，顯示在畜試土雞近親品系9與來亨雞品系P間的ESR基因型頻率存在品種間顯著的關聯性 (P<0.001)。雜交二代母雞產第1個蛋之日齡最早為127天，最晚為203天，平均168±14天。比較不同ESR基因型之母雞的產第1個蛋之日齡，顯示雜合型(EF)的母雞顯著地較純合型(EE 與FF)的母雞早熟(P<0.05)。
ASSOCIATION STUDY OF CHICKEN ESTROGEN RECEPTOR GENE AND EARLY MATURITY IN HEN
D. Y. Lin(1), Y. F. Lin(1), S. L. Hsing(1), H. L. Lin(1), Y. Y. Lai(1), H. L. Chang(2) and M. C. Wu(1)
(1)Livestock Research Institute, Council of Agriculture (2)Department of Animal Science, National Pingtung University of Science and Technology
F2 design reference family was set up a for chicken gene mapping, the F1 hybrid progenies was produced by reciprocal crosses between LRI native chicken inbred line 9( LRI-L9) and Leghorn, and F2 progenies was produced by fullsib mating of F1. Records of genotyping of DNA extracted from blood merged with the age at first egg (AFE) records was used to explore the association between maturity and genotype of estrogen receptor (ESR). Three genotypes (EE, EF and FF) of ESR were detected by PCR-SSCP. The genotype frequency of EE, EF and FF were 0%, 3.0% and 97.0% in LRI-L9, and 61.1%,33.3% and 5.6% in white Leghorn, respectively. The G0 data showed a strong association between breed and genotype (P<0.001). The average AFE of hens in F2 was 168±14 days. The Range of AFE of hens was 127 to 203 days. There was significant difference among genotypes in AFE of hens (P<0.05). Hens with heterotype(EF) of ESR were earlier mature than those hens with homotypes(EE and FF).
Key Words: Chicken, Maturity, Estrogen receptor gene