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53.民間羊場山羊黏多醣症基因頻率與基因多樣性分析

廖仁寶 陳若菁 陳水財 林秀蓮 吳明哲

行政院農業委員會畜產試驗所

山羊黏多醣症為一種隱性遺傳疾病，主要發生於努比亞品種。本研究自民間種羊場收集199 頭血液 樣品，包括阿爾拜因159 頭、撒能38 頭及吐根堡2 頭。經黏多醣症基因篩檢後，發現僅有三頭阿 爾拜因山羊之基因型為雜合型。進一步追查其系譜後發現此三頭羊含有努比亞血統。同時，亦應用 18 組微衛星型遺傳標記評估75 頭阿爾拜因山羊之基因多樣性，結果顯示其交替基因數、觀測異質 度(Ho)、期望異質度(He)及多態性訊息量(PIC)範圍分別為3–11、0.160–0.933、0.151–0.816 及 0.144–0.788，而其平均值則分別為7.2、0.671、0.656 及0.616，可得知此群阿爾拜因山羊的基因多 樣性具高度多態性資訊。

關鍵語：山羊、黏多醣症、基因多樣性

THE GENE FREQUENCY FOR CAPRINE MUCOPOLYSACCHARIDOSIS IIID AND GENETIC DIVERSITY OF GOATS FROM A PRIVATE GOAT FARM

R. B. Liaw, R. J. Chen, S. T. Chen, H. L. Lin and M. C. Wu

Livestock Research Institute, Council of Agriculture, Executive Yuan

Caprine mucopolysaccharidosis type IIID is a recessive inherited disorder which is mainly found in Nubian goats. One of the objectives of this study was to investigate the frequency of N-acetylglucosamine 6-sulfatase gene of goats from a private goat farm. A total of 199 goats including 159 Alpine goats, 38 Saan goats, and 2 Toggenburg goats were examined. The result indicated only 3 Alpine goats were detected as carrier genotype, and the three goats bear Nubian lineage by surveying their pedigrees. Furthermore, a total of 18 microsatellite markers were used to genotype 75 Alpine goat DNA for evaluating their genetic diversity. The results indicated that the values for allele number, observed heterozygosity (Ho), expected heterozygosity (He), and polymorphism information content (PIC) of the goats among all loci were in the range of 3–11, 0.160–0.933, 0.151–0.816, and 0.144–0.788, respectively. Moreover, the mean values for allele number, Ho, He, and PIC were 7.2, 0.671, 0.656, and 0.616, respectively. The result also indicated that the genetic diversity of the Alpine goats is high (PIC > 0.5).

Key Words: Goat, Mucopolysaccharidosis, Genetic diversity