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59.微衛星遺傳標記分析畜產試驗所白色絲羽烏骨雞的遺傳變異

林德育 劉曉龍 林義福 賴永裕 洪哲明 鄭裕信 吳明哲

行政院農業委員會畜產試驗所

為評估行政院農業委員會畜產試驗所白色絲羽烏骨雞產蛋數選育族群的遺傳變異。利用FAO(2004)建議使用的23組雞微衛星標記組分析87隻白色絲羽烏骨雞種雞個體DNA。其中除MCW0098、MCW0183及MCW0216三組微衛星標記所檢測的基因型在所有檢測個體皆為單型外，其它20組微衛星標記皆有多態型的基因型。共檢測到72個alleles，平均每個基因座具有3.1個對偶基因(1~7個alleles)；期望異質度介於0到0.819，平均為0.398；觀測異質度介於0到0.828，平均為0.372；多態性訊息含量平均為0.347。在本試驗選用的23組微衛星標記組中有7組呈現高度多態性資訊(PIC≥0.5)。8組呈現中度多態性資訊(0.5>PIC≥0.25)，8組呈現低度多態性資訊(PIC<0.25)。本試驗結果提供此白色絲羽烏骨雞選育族群遺傳變異之基本分子資訊。

關鍵語：白色絲羽烏骨雞、遺傳變異、微衞星標記

GENETIC VARIATION ANALYSIS OF LRI-COA WHITE SILKIE CHICKEN BY MICROSATELLITE MARKERS

D.Y. Lin, S.L. Liu, Y. F. Lin, Y.Y. Lai, C.M. Hung, Y.S. Cheng and M.C. Wu

Livestock Research Institute, Council of Agriculture

In order to evaluate genetic variation of white silkie chicken selection flock in Livestock Research Institute-Council of Agriculture(LRI-COA), Executive Yuan. A set of 23 microsatellite markers recommended for chicken in FAO’s DADIS MoDAD programme were utilized. The study was carried out on 87 birds from breeding flock to generate genotype data. Except 3 microsatellite markers, MCW0098、MCW0183 and MCW0216, all of the other microsatellites were polymorphic with average allelic number 3.1, ranged from 1 to 7 per locus. There were 72 alleles detected in total. The expected heterozygosity ranged from 0 to 0.819, and the average expected heterozygosity was 0.398. The observed heterozygosity of the population ranged from 0 to 0.828, and the average observed heterozygosity was 0.372. The estimated polymorphic information content (PIC) was 0347. In 23 markers, seven markers were highly informative with polymorphism information content (PIC ≥0. 50), eight markers were reasonably informative(0.5> PIC≥ 0.25) and the other eight markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of white silkie chickens in LRI-COA.

Key Words: White silkie chicken, Genetic variation, Microsatellite marker