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60.黑色絲羽烏骨雞之微衛星遺傳標記分析

林德育 劉曉龍 林義福 林秀蓮 洪哲明 謝昭賢 吳明哲

行政院農業委員會畜產試驗所

為評估行政院農業委員會畜產試驗所黑色絲羽烏骨雞族群的遺傳變異，利用FAO (2004)建議使用的23組雞微衛星標記組分析90隻白色絲羽烏骨雞種雞個體DNA。此23微衛星標記所檢測的結果皆有多態型的基因型。共檢測到79個alleles，平均每個基因座具有3.4個對偶基因(2~7個alleles)，其期望異質度介於0.125到0.746，平均為0.465；觀測異質度介於0.067到0.789，平均為0.448；多態性訊息含量平均為0.404。在本試驗選用的23組微衛星標記組中6組呈現高度多態性資訊 (PIC ≥ 0.5)，13組呈現中度多態性資訊 (0.5 > PIC ≥ 0.25)，4組呈現低度多態性資訊 (PIC < 0.25)。本試驗結果提供此黑色絲羽烏骨雞選育族群遺傳變異之基本分子資訊。

關鍵語：黑色絲羽烏骨雞、遺傳變異、微衞星標記

GENETIC VARIATION ANALYSIS OF LRI-COA BLACK SILKIE CHICKEN BY MICROSATELLITE MARKERS

D.Y. Lin, S.L. Liu, Y. F. Lin, H.L. Lin, C.M. Hung, C.H. Hsieh and M.C. Wu

Livestock Research Institute, Council of Agriculture

In order to evaluate genetic variation of black silkie chicken flock in Livestock Research Institute-Council of Agriculture (LRI-COA), Executive Yuan. A set of 23 microsatellite markers recommended for chicken in FAO’s DADIS MoDAD programme were utilized. The study was carried out on 90 birds from breeding flock to generate genotype data. All of the 23 microsatellites were polymorphic with average allelic number 3.4, ranged from 2 to 7 per locus. There were 79 alleles detected in total. The expected heterozygosity ranged from 0.125 to 0.746, and the average expected heterozygosity was 0.465. The observed heterozygosity of the population ranged from 0.067 to 0.789, and the average observed heterozygosity was 0.448. The estimated polymorphic information content (PIC) was 0404. In 23 markers, six markers were highly informative with polymorphism information content (PIC ≥ 0.50), 13 markers were reasonably informative (0.5 > PIC ≥ 0.25) and the other four markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of black silkie chickens in LRI-COA.

Key Words: Black silkie chicken, Genetic variation, Microsatellite marker