166

103.調查台灣黑豬場種豬之緊迫與多產基因頻率

陳佳萱 顏念慈 吳明哲

行政院農業委員會畜產試驗所

為協助台灣黑豬場進行基因選種，本試驗應用緊迫與多產基因檢測技術對7家台灣黑豬場，5個品種（高畜黑豬、梅山豬、桃園豬、六堆黑豬及雜交黑豬），共171頭種豬進行基因檢測。試驗結果顯示，在緊迫基因Hal-1843中，CC型（正常型）頻率分別為1.000、1.000、1.000、0.966及0.928，CT型（雜合型）頻率則為0.000、0.000、0.000、0.034及0.072，TT型(緊迫型) 頻率則為0.000、0.000、0.000、0.000及0.000；其中T緊迫基因頻率分別為0.000、0.000、0.000、0.017與0.036。在多產基因ESR中，AA型（非多產型）頻率分別為0.000、0.000、0.211、0.069及0.478，AB型（雜合型）為0.000、0.000、0.578、0.276及0.218，BB型（多產型）為1.000、1.000、0.211、0.655及0.304；其中B多產基因頻率分別為1.000、1.000、0.500、0.793及0.413。由試驗結果得知，在六堆黑豬與雜交黑中仍存在少數緊迫基因，而桃園豬與雜交黑豬在多產基因選種上仍有很大的發展空間，未來將持續協助業者將不良基因剔除以利後續黑豬選種。

關鍵語：黑豬、緊迫基因、多產基因

Investigation on the Frequencies of Halothane and Estrogen Receptor Genes of Breeding Pigs among Black Pig Farms in Taiwan

C. H. Chen, N. T. Yen, M. C. Wu

Livestock Research Institute, Council of Agriculture, Executive Yuan

In order to assist the gene selection of the Black pig farms in Taiwan, 171 breeding pigs, includes five breeds such as the KHAPS Black pigs, Meishan pigs, Taoyuan pigs, Liou-Duai Black pigs, and hybrid Black pigs, were used for preliminary investigating the gene frequency distribution of halothane (Hal-1843) and estrogen receptor (ESR) genes. The results showed that the CC, CT and TT (stress-sensitive type) genotype frequencies of Hal-1843 gene, were 1.000, 0.000 and 0.000 in KHAPS Black pigs; 1.000, 0.000 and 0.000 in Meishan pigs; 1.000, 0.000 and 0.000 in Taoyuan pigs; 0.966, 0.034 and 0.000 in Liou-Duai Black pigs; and 0.928, 0.072 and 0.000 in hybrid Black pigs, respectively. And the T allele frequency of Hal-1843 gene for KHAPS Black pigs, Meishan pigs, Taoyuan pig, Liou-Duai Black pig and hybrid Black pigs was 0.000, 0.000, 0.000, 0.017 and 0.036, respectively. In addition, the AA (non-prolific type), AB and BB (prolific type) genotype frequencies of ESR gene were, 0.000, 0.000 and 1.000 in KHAPS Black pigs; 0.000, 0.000 and 1.000 in Meishan pigs; 0.211, 0.578 and 0.211 in Taoyuan pigs; 0.069, 0.276 and 0.655 in Liou-Duai Black pigs; and 0.478, 0.218 and 0.304 in hybrid Black pigs, respectively. And the B allele frequency of ESR gene for KHAPS Black pigs, Meishan pigs, Taoyuan pigs, Liou-Duai Black pigs, and hybrid Black pigs was 1.000, 1.000, 0.500, 0.793 and 0.413, respectively. These results demonstrated that there still have stress-sensitive gene existed with low percentage in Liou-Duai and hybrid Black pigs, and there is massive potential for ESR gene selection in Taoyuan pigs and hybrid Black pigs. We will continually to help the Black pig industry to remove these unfavorable genes in the future.

Key Words: Black Pigs, Halothane Gene, ESR Gene