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中國畜牧學會會誌43(增刊) |
,2014 |
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J. Chin. Soc. Anim. Sci. 12(Suppl.) |
127
53.畜試土雞近親品系L12之微衛星遺傳標記多樣性分析
林德育(1) 曾淑貞(2) 林義福(1) 林秀蓮(1) 張秀鑾(3) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)國立屏東科技大學
為評估行政院農業委員會畜產試驗所畜試土雞台畜一號近親品系L12選育族群的遺傳變異，本試驗利用FAO(2004)建議使用的23組雞微衛星標記組，以及LEI0258微衛星標記組，共24組微衛星標記組分析該族群候選種雞80隻之個體DNA。24組微衛星標記皆有多態型的基因型。共檢測到65個對偶基因，平均每個基因座具有2.7個對偶基因(2~6個)，其期望異質度介於0.061到0.657，平均為0.40，觀測異質度介於0.038到0.888，平均為0.41，而多態性訊息含量平均為0.34。在選用的24組微衛星標記組中有6組呈現高度多態性資訊(PIC ≥ 0.5)，有11組呈現中度多態性資訊(0.5> PIC ≥ 0.25)，7組呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：土雞、遺傳多樣性、微衞星標記
GENETIC DIVERSITY ANALYSIS OF INBREEDING LINE L12 NATIVE CHICKEN IN LRI-COA BY MICROSATELLITE MARKERS
D. Y. Lin(1), S. J. Tzeng(2), Y. F. LIN(1), H. L. LIN(1), H. L. Chang(3) and M. C. Wu(1)
(1)Livestock Research Institute (LRI), Council of Agriculture
(2)Chung Hwa University of Medical Technology
(3)National Pingtung University of Science and Technology
In order to evaluate genetic variation of inbreeding line L12 native chicken flock in Livestock Research Institute-Council of Agriculture (LRI-COA), Executive Yuan. We use a set of 24 microsatellite markers to analyze 80 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 2.7, ranged from 2 to 6 per locus. The expected heterozygosity ranged from 0.061 to 0.657, and the average expected heterozygosity was 0.40±0.19 (mean ±SD). The observed heterozygosity of the population ranged from 0.038 to 0.888, and the average observed heterozygosity was 0.41±0.23. The estimated average polymorphic information content (PIC) was 0.34±0.16. In 24 markers, six markers were highly informative with polymorphism information content (PIC ≥0. 50), eleven markers were reasonably informative (0.5> PIC≥ 0.25) and the other seven markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population. Key Words: Native chicken, Genetic diversity, Microsatellite marker