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中國畜牧學會會誌46(增刊) |
,2017 |
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J. Chin. Soc. Anim. Sci. 46 (Suppl.) |
153
75.臺灣豐和黑毛烏骨雞之微衛星遺傳標記多態性分析
林德育(1) 曾淑貞(2) 王義松(3) 劉曉龍(1) 賴永裕(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)義松畜牧場
為評估臺灣豐和黑毛烏骨雞選育族群的遺傳變異，本試驗利用 FAO（2011）建議使用的 24 組雞微衛星標記組分析 48 隻臺灣豐和黑毛烏骨雞第 G5 世代種雞個體 DNA。24 組微衛星標記皆有多態型的基因型。共檢測到 89 個對偶基因，平均每個基因座具有 3.7 個對偶基因（2~9 個），其期望異質度介於 0.010 到 0.828，平均為 0.517，觀測異質度介於 0 到 0.688，平均為 0.408，而多態性訊息含量平均為 0.452。在選用的 24 組微衛星標記組中有 10 組呈現高度多態性資訊（PIC ≥ 0.5），有 11 組呈現中度多態性資訊（0.5> PIC ≥ 0.25），3 組呈現低度多態性資訊（PIC < 0.25）。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：黑毛烏骨雞、遺傳多樣性、微衞星遺傳標記
Genetic diversity analysis of TW Fengho black silkie chicken by microsatellite markers
D. Y. Lin(1), S. J. Tzeng(2), Y. S. Wang(3), S. L. Liu(1), Y. Y. Lai(1) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture
(2)Chung Hwa University of Medical Technology
(3)Yisong Livestock Farm
In order to evaluate genetic variation of TW Fengho black silkie chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 48 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 3.7, ranged from 2 to 9 per locus. The expected heterozygosity ranged from 0.010 to 0.828, and the average expected heterozygosity was was 0.517±0.187(mean±SD). The observed heterozygosity of the population ranged from 0 to 0.688, and the average observed heterozygosity was 0.408±0.213. The estimated average polymorphic information content (PIC) was 0.452±0.171. In 24 markers, ten markers were highly informative with polymorphism information content (PIC ≥0. 50), eleven markers were reasonably informative(0.5> PIC≥ 0.25) and the other three markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.
Key Words: Black silkie chicken, Genetic diversity, Microsatellite marker