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中國畜牧學會會誌46(增刊) |
,2017 |
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J. Chin. Soc. Anim. Sci. 46 (Suppl.) |
154
76.豐輝下營紅牌土雞之微衛星遺傳標記多態性分析
林德育(1) 曾淑貞(2) 楊豐輝(3) 賴永裕(1) 林秀蓮(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)豐輝牧場
為評估豐輝下營紅牌土雞選育族群的遺傳變異，本試驗利用 FAO（2011）建議使用的 24 組雞微衛星標記組分析 48 隻豐輝下營紅牌土雞第 G5 世代種雞個體 DNA。24 組微衛星標記皆有多態型的基因型。共檢測到 90 個對偶基因，平均每個基因座具有 3.8 個對偶基因（2 至 8 個），其期望異質度介於 0.118 到 0.822，平均為 0.492±0.202，觀測異質度介於 0 到 0.688，平均為 0.349±0.195，而多態性訊息含量平均為 0.434±0.194。在選用的 24 組微衛星標記組中有 6 組呈現高度多態性資訊（PIC ≥ 0.5），有 11 組呈現中度多態性資訊（0.5> PIC ≥ 0.25），7 組呈現低度多態性資訊（PIC < 0.25）。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：雞、遺傳多樣性、微衞星遺傳標記
Genetic diversity analysis of Fenghui Xiaying Red-Brand country chicken by microsatellite markers
D. Y. Lin(1), S. J. Tzeng(2), F. H. Yang(3), Y. Y. Lai(1), H. L. Lin(1) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture
(2)Chung Hwa University of Medical Technology
(3)Fenghui Breeder Farm
In order to evaluate genetic variation of Fenghui Xiaying Red-Brand country chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 48 candidate bred chickens from this flock. All the microsatellites were polymorphic. The average allelic number was 3.8, ranged from 2 to 8 per locus. The expected heterozygosity ranged from 0.118 to 0.822, and the average expected heterozygosity was 0.49±0.20. The observed heterozygosity of the population ranged from 0 to 0.688, and the average observed heterozygosity was 0.349±0.195. The estimated average polymorphic information content (PIC) was 0.434±0.194. In 24 markers, six markers were highly informative with polymorphism information content (PIC ≥ 0.50), eleven markers were reasonably informative(0.5 > PIC ≥ 0.25) and the other seven markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.
Key Words: Chicken, Genetic diversity, Microsatellite marker