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中國畜牧學會會誌47(增刊) |
,2018 |
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J. Chin. Soc. Anim. Sci. 47 (Suppl.) |
196
71.凱馨烏骨雞之微衛星遺傳標記多態性分析
林德育(1) 曾淑貞(2) 鄧學極(3) 賴永裕(1) 劉曉龍(1) 吳明哲(1)
(1)行政院農業委員會畜產試驗所 (2)中華醫事科技大學 (3)凱馨實業股份有限公司
為評估凱馨烏骨雞選育族群的遺傳變異，本試驗利用FAO(2010)建議使用的24組雞微衛星標記組分析64隻凱馨烏骨雞第G3世代種雞個體DNA。其中除MCW0103微衛星標記所檢測的基因型在所有檢測個體皆為單型外，其它23組微衛星標記皆有多態型的基因型。共檢測到98個對偶基因，平均每個基因座具有4.1個對偶基因(0~7個)；期望異質度介於0到0.798，平均為0.548；觀測異質度介於0到0.641，平均為0.400，而多態性訊息含量平均為0.491。在選用的24組微衛星標記組中有13 組呈現高度多態性資訊(PIC ≥ 0.5)，有9組呈現中度多態性資訊(0.5> PIC ≥ 0.25)，2組呈現低度多態性資訊(PIC < 0.25)。本結果提供此選育族群遺傳變異之基本分子資訊。
關鍵語：烏骨雞、多態性、微衞星遺傳標記
Polymorphism analysis of Kai Shing silkie chicken by microsatellite markers
D. Y. Lin(1), S. J. Tzeng(2), H. C. Teng(3), Y. Y. Lai(1), H. L. Liu(1) and M. C. Wu(1)
(1)Livestock Research Institute(LRI), Council of Agriculture
(2)Chung Hwa University of Medical Technology
(3) Kai Shing Trading Co., Ltd
In order to evaluate genetic variation of Kai Shing silkie chicken flock. we use a set of 24 microsatellite markers recommended by FAO to analyze 64 candidate bred chickens from this flock. Except MCW0103, all the microsatellites were polymorphic with average allelic number 4.1, ranged from 0 to 7 per locus. The expected heterozygosity ranged from 0 to 0.798, and the average expected heterozygosity was 0.548. The observed heterozygosity of the population ranged from 0 to 0.641, and the average observed heterozygosity was 0.400. The estimated average polymorphic information content (PIC) was 0.491. In 24 markers, 13 markers were highly informative with polymorphism information content (PIC ≥ 0.50), nine markers were reasonably informative(0.5 > PIC ≥ 0.25) and the other two markers were slightly informative (PIC < 0.25). These results could be provided basic molecular information for the research on the germplasm characteristics of the population.
Key Words: Silkie chicken, Polymorphism, Microsatellite marker