Embryo of Black Goat



Location of Preservation: Hengchun Research Station
Taiwan Livestock Research Institute
1 Ranch Road, Kenting
Hengchun, Pintung, Taiwan
Republic of China
Tel: 886-8-8861341~4
Fax: 886-8-8861345
Contributor: Mr. Ruey-Chun Hsieh


Equipments for Embryo Collection

Foley Catheter:

a. catheter
b. steel pole for insertion into the uterine horn
c. upper left-inlet for inflation of air bubble cuff
d. inlet for flushing and collecting embryo
e. inflatable cuff
Devices and Hormones for Induction of Estrus



Apparatus for Micromanipulation of Embryos

  1. Microscope for embryo examination
  2. Culture dish
  3. Pipette
  4. Media from left to right are: Intracellular cryoprotecting medium: 10% glycerol, 20% 1, 2-propanediol Extracellular cryoprotecting medium: 25% glycerol, 25% 1, 2-propanediol 1 M sucrose
  5. Straw


Programmable Freezer

  1. Program controller
  2. Incubation chamber
  3. Liquid nitrogen container with control valves and heater

Protocol of Embryo Cryopreservation

A. Collection of Embryo

  1. Induction of estrus:
    Method A: 1500 IU PMSG, 48 hours after, 2~3 ml PGF2a , 36~48 hours after, 1500 IU HCG intravenously. Often for breeding
    season.Method B: often for nonbreeding season day 1~9: CIDR(progesterone releasing device)in vagina day 7: 1500 IU PMSG intramuscularly day 9: Remove CIDR and PGF2a, injection day 10~11: 1500 IU HCG intravenoualy
  2. Breeding with artificial insemination or nature service
  3. Embryo collection on 6~7 days after mating
  4. Embryo examination