Embryo of Black Goat

Doe

Embryo


Location of Preservation: Hengchun Research Station
Taiwan Livestock Research Institute
1 Ranch Road, Kenting
Hengchun, Pintung, Taiwan
Republic of China
Tel: 886-8-8861341~4
Fax: 886-8-8861345
Contributor: Mr. Ruey-Chun Hsieh

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Equipments for Embryo Collection

Foley Catheter:

a. catheter
b. steel pole for insertion into the uterine horn
c. upper left-inlet for inflation of air bubble cuff
d. inlet for flushing and collecting embryo
e. inflatable cuff
Devices and Hormones for Induction of Estrus

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Apparatus for Micromanipulation of Embryos

  1. Microscope for embryo examination
  2. Culture dish
  3. Pipette
  4. Media from left to right are: Intracellular cryoprotecting medium: 10% glycerol, 20% 1, 2-propanediol Extracellular cryoprotecting medium: 25% glycerol, 25% 1, 2-propanediol 1 M sucrose
  5. Straw

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Programmable Freezer

  1. Program controller
  2. Incubation chamber
  3. Liquid nitrogen container with control valves and heater

Protocol of Embryo Cryopreservation

A. Collection of Embryo

  1. Induction of estrus:
    Method A: 1500 IU PMSG, 48 hours after, 2~3 ml PGF2a , 36~48 hours after, 1500 IU HCG intravenously. Often for breeding
    season.Method B: often for nonbreeding season day 1~9: CIDR(progesterone releasing device)in vagina day 7: 1500 IU PMSG intramuscularly day 9: Remove CIDR and PGF2a, injection day 10~11: 1500 IU HCG intravenoualy
  2. Breeding with artificial insemination or nature service
  3. Embryo collection on 6~7 days after mating
  4. Embryo examination

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