Contributor: Mr. Shang-Hsiang Wen, Mr. Kuang-Fuh LeeTaiwan Livestock Research Institute 1 Ranch Road,Kenting Hengchun, Pintung, Taiwan Republic of China Tel: 886-8-8861341~4 Fax: 886-8-8861345
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Portable electroejaculator (electricity rechargable), power switch on the right and electicity control button on the left.
Electric wire.
Electrode bar, the part inserted into the rectum.
Collected bar for penisto ejaculate and semen collection.
Centrifuge tube, semen collected.
Warm jacket, provide the 37 C temperature with water inside.
Straw powder, for sealing of the straw.
French straw (0.5 ml), for packaging semen.
Disposable syringe, to draw out and measure the volume of semen.
Straw rack, the straw will put on it then sitting on the place 5 cm above the level of liquid nitrogen bath to prefreeze.
a. Liquid nitrogen container for semen cryopreservation
b. Insulation container for semen prefrezing
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Semen Collection with Electroejaculator
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B. Preperation of Cryopreservation Solutions:
Solution A (without glycerol):
¡@154 ml sodium
citrate solution (2.9%)
¡@40 ml egg yolk
Solution B (with glycerol):
¡@132 ml sodium citrate solution (2.9%)
¡@40 ml egg yolk
¡@28 ml glycerol