Sperm of Yellow Cattle


Location of Preservation: Hengchun Research Station
Taiwan Livestock Research Institute
1 Ranch Road,Kenting
Hengchun, Pintung, Taiwan
Republic of China
Tel: 886-8-8861341~4
Fax: 886-8-8861345
Contributor: Mr. Shang-Hsiang Wen, Mr. Kuang-Fuh Lee


Equipments for Semen Collection

  1. Portable electroejaculator (electricity rechargable), power switch on the right and electicity control button on the left.

  2. Electric wire.

  3. Electrode bar, the part inserted into the rectum.

  4. Collected bar for penisto ejaculate and semen collection.

  5. Centrifuge tube, semen collected.

  6. Warm jacket, provide the 37 C temperature with water inside.

  7. Straw powder, for sealing of the straw.

  8. French straw (0.5 ml), for packaging semen.

  9. Disposable syringe, to draw out and measure the volume of semen.

  10. Straw rack, the straw will put on it then sitting on the place 5 cm above the level of liquid nitrogen bath to prefreeze.

a. Liquid nitrogen container for semen cryopreservation
b. Insulation container for semen prefrezing


Semen Collection with Electroejaculator


Protocol for Semen Cryopreservation

A. Semen Collection: With artificial vagina and electroejaculator

B. Preperation of Cryopreservation Solutions:

Solution A (without glycerol):
@154 ml sodium citrate solution (2.9%)
@40 ml egg yolk
Solution B (with glycerol):
@132 ml sodium citrate solution (2.9%)
@40 ml egg yolk
@28 ml glycerol

C. Microscopic Examination: Concentration, motility, abnormality.
Adding antibiotics into semen before examination.
D. Dilution:
1. Mix with semen and solution A in equal volume and equilibrate for at least two hours at 4 C.
2. Mix with diluted semen and solution B in equal volume and equilibrate for four hours at 4 C.
E. Strawing: Semen was drawed into the 0.5 ml French straw and then sealed with straw powder. Cooling the sealed straw in ice water for a while, and then taking out the straw and wipe them dry for prefreezing process.
F. Prefreezing: Straws were put on rack evenly and add the liquid nitrogen into the insulation container. Straws were 5 cm above the level of liquid nitrogen for freezing.
G. Frozen with Liquid Nitrogen: Straws were then directly put intoliquid nitrogen container for longterm preservation.