參考文獻

吳明哲、楊妙斐、陳坤照、陳鏡明。1996。馬GPIPGD遺傳型鑑別與G6PDLDH活性測定。畜產研究29(4): 279~288

黃瓊姿、鄭裕信、張秀鑾、李茂盛、戴 謙、陳晉蒼。1992。紐西蘭兔與雷克斯兔級進配種之繁殖與生長效果。畜產研究25(2): 125~130

張秀鑾、薛佑玲、吳松鎮、劉錦條、劉瑞珍、吳明哲。1994。種豬性能檢定豬PHI(GPI)PGD遺傳型鑑別。畜產研究27(2): 87~95

Andersson, L., R. K. Juneja and K. Sandberg. 1983. Genetic linkage between the loci for phosphohexose isomerase (PHI) and a serum protein (Xk) in horses. Anim. Blood Grps. Biochem. Genet. 14 : 45~50.

Fox, R. R. 1993. Linkage map of the rabbit (Oryctolagus Cuniculus) (2N=44). In Genetic Maps : Locus Maps of Complex Genomes. Book 4 : Nohuman Vertebrates (S. J. O’Brien ed., 6th Edition). pp. 4.258~4.263.

Gahne, B. and R. K. Juneja. 1985. Prediction of the halothane (Hal) genotypes of pigs by deducing Hal, Phi, Po2, Pgd halotypes of parents and offspring : Results from a large scale practice in Swedish Breeds. Anim. Blood Grps. Biochem. Genet. 16 : 265~283.

Yerle, M., A. L. Archibald, M. Dalens and J. Gellin. 1990. Localization of PGD and TGFB-1 to pig chromosome 6q. Anim Genet. 21 : 411~417.

 

Nz.jpg (24289 個位元組)

1. 紐西蘭白兔。
Fig. 1. New Zealand White rabbit.

 

rws.jpg (17616 個位元組) rcs.jpg (20261 個位元組)
rccs.jpg (16895 個位元組) ros.jpg (20966 個位元組)

2. 雷克斯兔的四個毛色品種:白色(W)、海狸色(C)、錦企拉色(CC)和寶藍色(O)
Fig. 2. Four fur color breeds of Rex rabbit : White (W), Castor (C), Chinchilla (CC) and Opal (O).

 

rf3.jpg (46227 個位元組)

3. 濾紙採血樣步驟:(a)採血時先針刺血管,(b)採樣用濾紙片,(c)吸血樣,(d)放入個別的保存管。
Fig. 3. Protocols for blood sampling by using filter paper are: (a) bleeding by syringe needle, (b) filter paper applying, (c) blood absorbing with filter paper, and (d) preserving sampled paper in individual tube.

 

rf4.jpg (35030 個位元組)

4. 剪片溶血與吸取融解血樣。
Fig. 4. A piece of sampled filter was cut into the solution to have the blood sample for testing.

 

rf5.jpg (34881 個位元組)

5. 兔的GPIPGD電泳相及遺傳型判讀。血樣排列由上而下有馬、馬、兔、兔、豬、豬、豬等七個。GPI的舊稱是PHI
Fig 5. Genotyping of GPI and PGD based upon the banding type after electrophoresis in rabbit. Seven blood samples applied from top to bottom were horse, horse, rabbit, rabbit, pig, pig and pig. The old name for GPI was PHI.

 

1. 各品種之GPIPGD遺傳型頻率

Table 1. Distribution of GPI and PGD genotypes by breed

Breed

No. of rabbits

Genotype

GPI

PGD

AA

AB

BB

AA

AB

BB

ˍˍˍˍˍˍˍˍˍˍˍˍˍˍ

New Zealand White

172

0

0

100.0

70.9

23.9

5.2

White Rex

56

0

0

100.0

100.0

0

0

Castor Rex

65

0

0

100.0

100.0

0

0

Chinchilla Rex

266

0

0

100.0

100.0

0

0

Opal Rex

48

0

0

100.0

100.0

0

0

 

2. 紐西蘭白兔群的子代PGD遺傳型頻率

Table 2. Distribution of PGD genotypes in progeny of New Zealand White stocks

Mating type

Genotype of progeny

Sire

Dam

AA

AB

BB

AA

AA

340(100.0)

   

AA

AB

105( 61.0)

67( 39.0)

 

AA

BB

 

14(100.0)

 

AB

AA

31( 54.0)

30( 46.0)

 

AB

AB

13( 34.0)

14( 37.0)

11(29.0)

AB

BB

 

1( 50.0)

1(50.0)

Overall

489( 78.0)

126( 20.1)

12( 1.9)

Value in parentheses is the corresponding frequency.